Use of soft materials for enzyme immobilization

Enzymes are typically immobilized for easy separation. Often, this takes place on (or in) soft materials such as alginate or agarose beads, or cross-linked enzyme aggregates. These materials tend to have a low tolerance for pressure gradients, creating challenges for the fixed bed reactor. The stirred tank reactor will quickly damage the soft materials due to their poor mechanical strength.

SpinChems' Rotating Bed Reactor (RBR) solves the issue of mechanical damage and circumvents the high pressure drops, giving researchers a tool to develop novel methods in advanced materials.

Below are few applications where researchers have used RBR with soft materials for immobilization of enzymes:

  • The RBR was setup for in-situ formation, loading and handling of alginate beads were demonstrated. Results show the use of the rotating bed reactor allowed easy collection, maturing and washing of the alginate beads. It was possible to reduce the number of handling steps and facilitating bead recycling. The beads showed no signs of physical wear after use in the RBR.

  • In research performed at Indian Institute of Technology Hyderabad, the catalytic activity of L-asparaginase. was increased by nearly 60%  by immobilization of the enzymes in alginate beads, and by using a rotating bed reactor the mass transfer limitations were reduced.
  • Production of recombinant choline oxidase and its application in betaine production with the immobilized choline oxidase in the RBR was performed, reaction conditions such as pH and temperature were optimized and the enzymatic activity was measured for the reaction of choline to glycine betaine via betaine aldehyde.

  • Production of hydroxytyrosol rich extract from Olea europaea leaf with enhanced biological activity using RBR was experimented where the immobilized enzyme showed good stability over time, the enzyme beads were damaged mechanically in the STR and could not be re-used. The RBR solved the problem of bead attrition allowing the immobilized enzyme to be re-used and thus giving superior total turnover number to the STR.

  • Efficient synthesis of chiral lactones by encapsulated cells in a rotating bed reactor. Comparison of SpinChem® rotating bed reactor with traditional reaction set-ups for a demanding biotransformation was performed. SpinChem® RBR matched or outperformed the other systems and gave a 10 to 25-fold more time-efficient recycling of the encapsulated cells.

Thus, the Rotating Bed Reactor (RBR) from SpinChem addresses the problem of mechanical damage and avoids significant pressure dips, providing a platform to create new procedures in advanced materials.

What is holding you back from an efficient and economically sustainable process? Learn more about different RBRs and its technology. Get in touch with us today.

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